KMID : 0870420040080030172
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Korean Journal of Hepato-Biliary-Pancreatic Surgery 2004 Volume.8 No. 3 p.172 ~ p.179
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Cyclooxygenase-2 Expression in Bile Duct Cancer
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Her Kyu-Hee
Kim Sun-Whe Jang Jin-Young Yoon Yoo-Seok Kim Kwang-Sig Lee Hye-Seung Yoon Yong-Bum Park Yong-Hyun
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Abstract
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Purpose: Epidemiological studies have shown that regular ingestion of a nonsteroidal anti-inflammatory drug reduces the risk of fatality from colon cancer. Cyclooxygenase-2 (COX-2) has been found to be overexpressed in various types of tumor, including colorectal cancer. It has been suggested that the COX-2 enzyme may play an important role in carcinogenesis and tumor progression. The aims of this study were to find the relationship between COX-2 expression and bile duct carcinogenesis and its clinical significance in bile duct cancer.
Methods: The COX-2 expression was determined using three methods, reverse transcription polymerase chain reaction (RT-PCR), Western blotting and immunohistochemical staining. mRNA and proteins were extracted from the tissue specimens of 22 bile duct cancer patients.
Results: RT-PCR and western blotting found COX-2 expressions in all the cancers and their paired noncancerous tissues. Immunohistochemistry revealed the highest levels of COX-2 expressed in bile duct carcinoma cells, mainly in the cytoplasm, and a weak reactivity of the COX-2 protein was observed in noncancerous bile duct epithelial cells.
Conclusion: Based on this study, it is postulated that the carcinogenesis mechanism of COX-2 expression may be initiated from a noncancerous condition, such as chronic inflammation, and may advance to bile duct cancer progression.
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KEYWORD
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Bile Duct Neoplasms, Cyclooxygenase, Immunohistochemistry, Western Blotting
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